Despite significant variability among clones, western blot analysis of Sirt1 +/+ versus Sirt1 −/− iPSCs at passage 35, when the divergence in telomere length is more pronounced, showed a clear trend toward decreased mTERT expression in Sirt1 −/− iPSCs compared to wild-type ( Figures 5 A and 5C, left panels).
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SIRT1 is necessary for proficient telomere elongation and genomic stability of induced pluripotent stem cells.
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