Gene pleiotropy constrains gene expression changes in fish adapted to different thermal conditions.

In line with this finding, we observed a weakly significant association between GO-CC and both plastic and evolutionary gene expression responses ( Supplementary Figs 3 and 4 and Supplementary Data 1 and 2 ).

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We found a highly significant effect of temperature treatment on protein expression profiles and a significant effect of thermal origin but no interaction between them ( Fig. 3 and Supplementary Fig. 1 ).

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