Experiments in additional hPSC lines verified that our monolayer differentiation protocol, consisting of BMP4/GSK-3βI administration in phase 1 and VEGF-A/DAPT administration in phase 2, resulted in robust endothelial differentiation with minimal differences in efficiency between different cell lines that did not reach statistical significance ( Supplementary information, Figure S2F ).
← all excerpts
Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells.
1
—
—