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Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells.

Cell Res · 2014 · PMC4085760 · PMID 24810299

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Experiments in additional hPSC lines verified that our monolayer differentiation protocol, consisting of BMP4/GSK-3βI administration in phase 1 and VEGF-A/DAPT administration in phase 2, resulted in robust endothelial differentiation with minimal differences in efficiency between different cell lines that did not reach statistical significance ( Supplementary information, Figure S2F ).

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