Comparison of reconstructions based on FI or biocytin histology (BH) revealed a significant trend for those created using 2PLSM/FI to have larger process diameter than those based on BH (Figure 4 ).
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A comparison of manual neuronal reconstruction from biocytin histology or 2-photon imaging: morphometry and computer modeling.
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Interestingly, whilst models based on FI reconstructions exhibited a small trend for smaller depolarizations, this was indistinguishable between methods at all locations (Figure 5B ).