Fasting increases human skeletal muscle net phenylalanine release and this is associated with decreased mTOR signaling.

Eukaryotic factor 2 subunit α (eIF2α Ser 51 phosphorylation showed significant interaction (p = 0.048) between fasting and insulin stimulation after the 72-hour-fast and post-hoc testing showed a trend (p = 0.059) toward decreased phosphorylation after 72 hours of fasting ( Figure 3H ).

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