Reference values of whole-blood fatty acids by age and sex from European children aged 3-8 years.

44 Our data confirmed a decreasing trend of C22:5n-3 with age in girls but we found C18:2n-6 to increase with age and no age dependence for C20:4n-6.

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A positive age trend of C18:2n-6 or a negative trend of C20:5n-3, as observed in our sample, was not reported by Harris et al. , but their sample may not be comparable to ours because of older age groups and the selection of patients whose blood samples were analysed for diagnostic purposes.

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C18:0 and C18:2n-6 showed a positive trend with age, whereas this trend was negative for C16:0, C18:1n-9 and C20:5n-3 in both boys and girls ( Figure 2 ).

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