only marginally significantP = 0.083
The knockdown for CG4836 showed no significant downregulation compared with Curly flies carrying only the responder (t 6 = 0.80; P = 0.231) and CG14891 was only marginally significant (t 7 = 1.77; P = 0.083), with all other gene knockdowns showing significant reductions in gene expression relative to controls ( Figure 4 ).