Of five cell lines tested (IGROV1 cells did not form spheroids at all), we observed significantly increased numbers of spheroids in the SP fraction of four models (i.e., A2780, A2780V, B16/92, B17/92), whereas in the fifth cell line (i.e., B2/92) we could only detect a slight trend that did not reach statistical significance (Fig. 3C + 3D).
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The side population of ovarian cancer cells defines a heterogeneous compartment exhibiting stem cell characteristics.
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