Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.

342 , 343 Nowadays, an increasing trend is to seek relevant diversity, aligned using tools such as Clustal Omega, 344 , 345 MUSCLE, 346 PROMALS, 347 , 348 or other methods based on polypharmacology, 141 , 349 , 350 that one may hope contains enzymes capable of effecting the desired reaction.

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We detect a significant trend towards more of the latter, 519 for instance in the use 368 , 1235 , 1296 , 1297 of molecular dynamics to calculate properties that suggest which residues might be creating internal friction 1298 , 1299 and hence lowering k cat .

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