The 1.5 × IQR values were calculated and the cut-offs set at 1.56 for the 10276 WT/10276 bsaZ:: pDM4 data set, 1.79 for the 10276 bipD:: pDM4 / 10276 bsaZ:: pDM4 data set, and 1.46 for the 10276 Δ bsaP/ 10276 bsaZ:: pDM4 data set, based on the calculation described under “Experimental Procedures.” When the data was plotted as a boxplot, the data showed a trend toward more proteins with larger ratios in the 10276 bipD:: pDM4 / 10276 bsaZ:: pDM4 and 10276 Δ bsaP/ 10276 bsaZ:: pDM4 data sets when compared with the 10276 WT/10276 bsaZ:: pDM4 data set ( Fig. 2 A ).
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Quantitative proteomic analysis of Burkholderia pseudomallei Bsa type III secretion system effectors using hypersecreting mutants.
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