Conversely, BRCA1 Δ11/Δ11 MEFs overexpressing RNF168 R57D or 53BP1 DN had less PARPi-induced chromosomal aberrations than control cells, although in the case of RNF168 R57D the difference did not reach statistical significance (Figure 3B and C ).
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Ectopic expression of RNF168 and 53BP1 increases mutagenic but not physiological non-homologous end joining.
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