showed a trendP = 0.054
However, the colocalization of GnRH and vGluT2 punta density only showed a trend for a decrease (P = 0.054) that, together with the different ages at which GnRH (young to old) and vGluT2 (young to MA) decreased, suggests that the age-related decline of vGluT2 in the median eminence was probably not associated specifically with the GnRH system.