Microspore culture reveals complex meiotic behaviour in a trigenomic Brassica hybrid.

Fertility as measured by either pollen viability or seed set was not significantly associated with DNA ploidy level in any population ( p > 0.05), with the exception of a weakly significant association ( p = 0.043, r 2 = 0.07) between ploidy and pollen viability in the MD population.

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By contrast, univalent chromosomes B j 3 and B j 7 both showed highly significant deviation from expected transmission ratios: B j 3 was preferentially retained (~95 % of the time) and B j 7 was preferentially lost (present 25 % of the time) (Additional file 4 : Figure S2).

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