Although there was a strong correlation with the expected values, linear regression showed a clear trend for the bisulfite amplicon NGS to over-estimate the percent methylation (intercept of + 8%, Figure 5 A,B), evident in sample of mixed ratios by 1.32-fold on average.
← all excerpts
Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis.
1
—
—