Electroporation Knows No Boundaries: The Use of Electrostimulation for siRNA Delivery in Cells and Tissues.

6 When sequences within the genome show partial complementarity to the seed region (nucleotides 2–8), the siRNA/shRNA can exert quite significant off-target effects. 7 , 8 Computational methods and chemical modifications to the siRNA oligonucleotides have made significant improvements to reduce off-target silencing. 9 The efficiency of RNAi also depends on multiple other parameters such as the half-life of the mRNA, the amount of mRNA, and the localization in the cell.

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