Nitrative stress, oxidative stress and plasma endothelin levels after inhalation of particulate matter and ozone.

Plasma oxidative stress markers Changes of plasma m-tyrosine levels after exposure to air contaminants did not reach statistical significance (Fig. 6a ), while o-tyrosine (Fig. 6b ; 3-way ANOVA, Ozone × EHC-93, p = 0.025; 2-way ANOVA, Ozone × EHC-93, p = 0.040) and p-tyrosine (Fig. 6c ; 3-way ANOVA, Ozone × Recovery, p < 0.001, EHC-93 main effect, p < 0.001; 2-way ANOVA, EHC-93 main effect, p = 0.010) were affected by both pollutants.

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Although these particles do not cause pulmonary inflammation, interestingly there was an increasing trend in total BAL cells immediately post exposure when the animals were exposed to particles alone which may have led to increase in iNOS [ 37 ] and thus NO• production in lung.

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