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Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana.

Plant Methods · 2015 · PMC4607171 · PMID 26472987

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showed a trendno p-value reported
tabacum leaves and their experiments showed a trend of increased transient expression towards the basal parts of the leaf.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.