By contrast, Heat shock protein 68 ( Hsp68 ), which is a gene of the stress-inducible HSP 70 group [ 21 ], showed a trend for downregulation at 12 h post-cohabitation in N W as well as in W W beetles, whereas W N beetles showed Hsp68 upregulation potentially as a direct result of wounding.
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Downregulation of the evolutionary capacitor Hsp90 is mediated by social cues.
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Our data confirm and support the results of the first experiment, showing a persistent and highly significant reduction of Hsp83 expression in both males and females from the N W group relative to the N N group, which was independent of mating ( figure 3 ).