Interestingly, there was a clear trend of Cdc42 reaching the maximum intensity value before GRAF1 (on average 5 s before the GRAF1 intensity maximum, and 3 s after the start of the GRAF1 peak) ( Fig. 4 E).
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Endocytic membrane turnover at the leading edge is driven by a transient interaction between Cdc42 and GRAF1.
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