The destabilizing effect of a mismatch is depending on the base pair type with a clear trend for the order of decreasing stability: G-C > A-T > G·G > G·T ≥ G· A > T·T ≥ A·A > T·C ≥ A· C ≥ C·C [ 33 ].
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Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays.
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