A clear trend is that at the low solvent temperatures the protein has higher numbers of internal HBs than at the high solvent temperatures, although lowering the protein temperature (when the solvent temperatures remain unchanged) can still increase the HB number, e.g., 6.3 from P300/S180 to P180/S180 and 13.4 from P300/S300 to P180/S300.
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Effect of the Solvent Temperatures on Dynamics of Serine Protease Proteinase K.
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