When assessed for the fraction of second pulse replication origins with and without TPT treatment, NUCKS1-depleted cells showed a trend toward increased levels of second pulse replication origins, while RAD51AP1-depleted cells significantly suppressed firing of second pulse replication origins after TPT treatment (Figure 6G ).
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NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability.
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