Refinement of atomic models in high resolution EM reconstructions using Flex-EM and local assessment.

The refined model has quite significant variations from the model deposited with the map, with Cα RMSDs of 4.53 Å, 5.36 Å and 5.19 Å for the equatorial, intermediate and apical domains. 3.1.4 GroEL (3.3 Å resolution) To compare model refinements carried out on raw and sharpened maps, we used an E. coli GroEL map in the apo-form, reconstructed at 3.3 Å resolution ( Suppl methods ).

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