near-significantp = 0.053
Although the extent of the effect of Rap1 CA on PKBR1 phosphorylation varied between experiments, likely due to varying levels of Rap1 CA expression related to plasmid copy number per cell as was previously described 25 26 27 , quantification of the data nonetheless revealed significant (at 40 and 60 sec) or near-significant effects (5 sec, p = 0.053; 10 sec, p = 0.084; 20 sec, p = 0.077).