Conditions Tested Lo-Bind eppendorfs versus standard microfuge tubes Multiple freeze/thaw cycles Retrieval: Schirmer strip versus microcapillaries versus minisponges Different tear volumes (1μl, 3μl, 5μl) Sample storage (-20 °C versus -80 °C) Results: Multiple freeze/thaw cycles showed significantly reduced levels of IL1-β and -2 and showed a trend for a reduction in the other 5 cytokines.
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