Differential effects of male nutrient balance on pre- and post-copulatory traits, and consequences for female reproduction in Drosophila melanogaster.

an increase in male attractiveness; Dispersion: 0.333; Carbohydrate: F 1,104 = 3.044, p-value = 0.084; Table S2 ), which measures male attractiveness when focal males encounter an unreceptive females; there was also a borderline non-significant interaction protein*carbohydrate on the latency of females to mate with the focal male (Protein*Carbohydrate: F 1,99 = 3.799, p-value = 0.054, Table S2 ).

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P1 experiment – focal male 1 st to mate Dietary effects on pre-copulatory traits There was a marginally non-significant quadratic effect of focal male protein intake on our measure of male attractiveness to virgin females (the latency of virgin females to mate with the focal male), in which the nutritional landscape peaked at an intermediate protein intake of ~500 μg and a low-to-intermediate carbohydrate consumption (~150 μg to ~1500 μg; Dispersion: 0.218; Protein*Protein: F 1,206 = 3.454, p-value = 0.064; Table S2 ) with P:C ratios lying between 3:1 and 1:3 ( Fig. 3a ).

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