However, this elevation did not reach statistical significance in any of the genes investigated, except for the Sycp3, where the plain culture setting (only MEMα + KSR 10% (v/v)) had a significantly upregulated Sycp3 expression compared to the control pre-pubertal 5 d pp rat testis ( Supplementary Figure 2 ).
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In vitro differentiation of rat spermatogonia into round spermatids in tissue culture.
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