In the absence of H 2 O 2 , cold stimulation (from 26 to 16 °C) failed to increase the NP O of hTRPA1-WT, but significantly increased the NP O of hTRPA1-P394A compared with the basal level (26 °C), although it did not reach statistical significance when compared with the NP O of hTRPA1-WT at 16 °C.
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Cold sensitivity of TRPA1 is unveiled by the prolyl hydroxylation blockade-induced sensitization to ROS.
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