approached significanceP = 0.064
A paired samples t-test showed that the difference in the median values of all fluorescence and scatter profiles at the removal of nitrogen and again at the end of gametogenesis, was significant only for the channel FL1 (t 1 = 16.0 P = 0.040) and approached significance for side-scatter (t 1 = 9.9 P = 0.064) in this small late logarithmic dataset.