E2F1 controls germ cell apoptosis during the first wave of spermatogenesis.

A significant decrease in the number of MAGE‐B4‐positive cells was observed at P10, although the decline in spermatogonial proliferation did not reach statistical significance (Fig. 5 B).

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In the histological evaluation and the flow cytometric analysis it was seen that a highly significant proportion of the primary spermatocytes were lost and the number of round spermatids was consequently decreased in the E2F1 −/− ‐testis at P20.

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