Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner.

After 4 months of exposure, mice exposed to nicotine-containing e-cigarette fluids showed a trend towards increased cell number in BALF ( figure 2 A).

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In cells exposed to 10 μM nicotine in the culture media, CBF decreased from 7.2±0.3 in controls to 6.2±0.3 (mean±SEM; p<0.05; figure 8 B) and there was a decreasing trend in the CFTR activity ( figure 8 C).

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