Enhanced Human-Induced Pluripotent Stem Cell Derived Cardiomyocyte Maturation Using a Dual Microgradient Substrate.

Additionally, the aspect ratio (AR) (depth/spacing), like used in previous studies, 27 , 28 is plotted against the orientation, eccentricity, and elongation of hiPSC-CM in Figures 6 A, 6 B and 6 C , respectively. In those figures, a clear trend can be observed where the increase of the AR results in a higher percentage of aligned cells ( Figure 6 A), and cells with a higher eccentricity ( Figure 6 B) and elongation ( Figure 6 C).

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