In addition, DHA-containing PSs and PAs, such as PS40:6(18:0/22:6), PS40:7(18:1/22:6) and PA38:6(16:0/22:6) were negatively correlated ( r = −0.6 to −1) with VLC-SMs and VLC-Cers, as well as glycosylated sphingolipids including GalCers, LacCers, and SLs; that were approaching significance ( p < 0.1); while monounsaturated PAs including PA34:1(17:0/17:1), PA36:1(18:0/18:1) and PA36:1(16:0/20:1) were positively correlated ( r = 0.8 to 1, p < 0.05).
Nonetheless, we have performed correction for false positives using the q values [ 47 ], which shown that most of the reported lipidomic changes have remained significant at q < 0.05 or marginally significant at q < 0.1 even after correction for multiple comparisons ( Supplemental Tables S2-4 ), vindicating the validity of the proposed gerotargets and pathways in the current study.
Specifically, the authors found that PUFA generally sharply decreased with age up to 18 years old, following which the decline is either attenuated or much more gradual; while DHA 22:6 exhibited an increasing trend with a peak in abundance at approximately 18 years, setting itself apart from all remaining PUFAs (C22:5, C22:4, C20:4, C20:3) examined [ 7 ].