For the CI+LZ+ MYPT1 isoform, GTPγS treatment increased Thr696 phosphorylation, but the increase did not reach statistical significance ( Fig. 3 ), which could suggest the CI domain modulates Rho kinase phosphorylation.
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MYPT1 isoforms expressed in HEK293T cells are differentially phosphorylated after GTPγS treatment.
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