Fungal diversity and seasonal succession in ash leaves infected by the invasive ascomycete Hymenoscyphus fraxineus.

Biotrophs ( Exobasidium , Phyllactinia ) generally showed a trend of increase in read proportions in the spore material towards the end of the sampling period, which was in line with their increased read proportions in leaf tissues towards autumn.

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fraxineus showed significant increases in ITS‐2 read percentages in leaflets and petiole base, the biotrophic genus Exobasidium showed significant increase in ITS‐1 and ITS‐2 read proportions in leaflets, while the biotrophic genus Phyllactinia showed a nonsignificant trend of increase in ITS‐1 and ‐2 read percentages in leaflets.

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