Immunogenicity of oncolytic vaccinia viruses JX-GFP and TG6002 in a human melanoma in vitro model: studying immunogenic cell death, dendritic cell maturation and interaction with cytotoxic T lymphocytes.

There was no significant difference between controls and TG6002-treated cells, although we observed a higher HMGB1 release upon TG6002 incubation, which was close to significance ( P =0.059) ( Figure 4A ).

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The combination of viral infection and either 5-FC or 5-FU did further increase the expression of calreticulin in case of SK29-MEL-1 cells but did not reach statistical significance too ( Figure 4B , left part).

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