Towards a new standardized method for circulating miRNAs profiling in clinical studies: Interest of the exogenous normalization to improve miRNA signature accuracy.

We went on to show that the use of exogenous normalizers only for absolute RT‐qPCR concentrations measurements did not influence the correlation with microarray data (standard normalization) for miR‐16‐5p (r = 0.52; P = 7.1E‐2) and miR‐486‐5p (r = 0.84; P = 2.9E‐4), although the correlation becomes slightly significant for miR‐93‐5p (r = 0.55; P = 4.9E‐2) ( Figure 4 middle panel).

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