Gene length and detection bias in single cell RNA sequencing protocols.

For all three full-length protocol datasets, shorter genes have lower count level expression proportions compared to longer genes, with a clear trend of increasing log-counts as gene length increases ( Figure 1a, d, g ).

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This was accompanied by a decreasing trend in the dropout rate per gene as gene length increased, highlighting the fact that shorter genes are more difficult to detect using full length protocols ( Figure 1b, e, h ).

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