Evidence that Mediator is essential for Pol II transcription, but is not a required component of the preinitiation complex in vivo.

As stated in point 4 of new experiments, our statistical analysis demonstrates that the altered pattern is highly significant (although the Med17 result is less clear). 6) The low Mediator:Pol II ratio observed in strains depleted of both a Mediator subunit and Kin28 represents our most direct evidence supporting the conclusion that Mediator is not a required component of the PIC.

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As was qualitatively clear from the graphs, the downstream shifts observed in Med22, Med14, and Med7 depletion strains are clearly significant, whereas no downstream shift is observed in Med15 and Cdk8 depletion strains.

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