Another main growth factor involved in angiogenic processes is angiopoietin 1, which showed a trend of upregulation in all conditions (ranging from a fold change of 4 to 7 in the different culture conditions, p > 0.05) at day 14 ( Figure 6(b) ).
A slight trend of increase in measured green fluorescent signal in the entire image was detected in all conditions by day 3 ( Figure 5(a) ), raising from 28.7 ± 6.9% to 46.9 ± 16.4%, from 28.4 ± 5.2% to 43 ± 3.3%, and from 27.3 ± 7% to 40.9% in 100 H, 75 HM, and 50 HM cultures, respectively (all p > 0.05).