Barely Significant
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Nucleotides upstream of the Kozak sequence strongly influence gene expression in the yeast <i>S. cerevisiae</i>.

J Biol Eng · 2017 · PMC5563945 · PMID 28835771

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Two point mutations—cytosine ( k 51 ) and guanine ( k 53 )—resulted in statistically significant differences in fluorescence from k 1 , whereas a thymine ( k 52 ) augmented k 1 fluorescence by about 14 % but this did not reach statistical significance.

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