The loss-of-function allele Hiw ND8 (Hiw↓) or the overexpression of a form deleted in the E3 ubiquitin ligase domain, Hiw Δ3 , yielded only a trend towards synapse number reduction that failed to reach statistical significance ( Fig 3A ).
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The equilibrium between antagonistic signaling pathways determines the number of synapses in Drosophila.
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