There was no significant increase in the UCP1 mRNA level after 30 minutes of treatment with ANP (Figs 5b,d ), a time point at which the ANP-induced intracellular temperature increase did not reach statistical significance (Fig. 4 ).
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The thermogenic actions of natriuretic peptide in brown adipocytes: The direct measurement of the intracellular temperature using a fluorescent thermoprobe.
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