Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues.

Sci Rep · 2017 · PMC5645415 · PMID 29042640

Europe PMC DOI

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0.0770

closest p · 1.5× alpha

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boldest claim

The sentences

a significant trendP = 0.077so close (0.05 < p ≤ 0.1)

The correlation analysis showed that the more reliable consistent markers in terms of density reproducibility between each mIF batch and chromogenic IHC included PD-L1, CD3, CD4, and PD-1, followed by CD8 and CD57, which showed a significant trend (r = 0.555, P = 0.077; r = 0.527, P = 0.096, respectively) in the second batch.

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