showed a trendP < 0.01
Western blot analysis—In neonatal, ENDO showed a trend of expression, characterized by a significant increase from the inner to outer part of the meniscus (Fig. 3 , P < 0.01), while young and adult animals revealed no quantitative differences among the different meniscal areas (Fig. 3 , P > 0.05); furthermore, pooling the three zones, the young meniscus revealed a significantly higher expression of ENDO in comparison with neonatal and adult ones ( P < 0.01).