DNA content assay The assessment of DNA remaining in the ECM after the decellularization processes showed a trend to less DNA content in the samples processed according to Protocol II, in both maternal (412 ng/mL) and fetal portions (418 ng/mL) compared to Protocol I, which presented DNA concentrations of 891 ng/mL and 1,125 ng/mL, in maternal and fetal portions respectively ( Figure 5 ).
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