Within the apoptotic pathway, Bim had borderline significance when compared to baseline at 24-hrs ( p = 0.03) and had a significant Trend Test ( p = 0.005), phospho-BAD (S112) was statically significant at 1-hr ( p = 0.009), at 5 h ( p = 0.005), and 24 h ( p < 0.001). phospho-BAD (S155) was also statically significant at 5 h ( p = 0.003) and 24-hrs ( p = 0.007) while phospho-FADD (S194) was borderline at 5 h ( p = 0.03) and significant at 24 h ( p = 0.001).
Shown in the expanded CScape view are expression of phospho-mTOR (S2448), which was significantly increased at 5 h ( p = 0.005) and 24 h ( p < 0.001), phospho-p70 S6 Kinase (S371) with borderline significance at 5 h ( p = 0.02) and significance at 24 h ( p < 0.001), phospho-eIF4G (S1108) with borderline significance at 5 h ( p = 0.02) and significant at 24 h ( p < 0.001). phospho-GSK-3a/B (S21/9) was significantly different at 5 h ( p = 0.002) and 24 h ( p = 0.001), expression of p21 Waf1/Cip1 was significant at 24 h ( p = 0.001), p27 Kip1 ( p = 0.012) was significant at 24 h, phospho-PAK1/2 (T423/402) was significant at 24 h ( p = 0.009), phospho-MDM2 was significantly different at (S166) at 5 h ( p < 0.001) and 24 h ( p < 0.001), phospho-Cyclin D1 (T286) was significant at 24 h ( p = 0.01).
Within the MAPK pathways (Table 1 ), phospho-epidermal growth factor receptor (EGFR) (Y1068) was borderline significant at 1 h ( p = 0.02) and statistically significant at 5 h ( p = 0.004).
There were four proteins with marginal significance ( p ≤ <0.05) in any of the time points, but the Trend Test was statistically significant ( p ≤ 0.0125).