Comparison of the phenotype of the Adamts5 -/- -J mice of the present study with that of the Adamts5 -/- -P mice reveals some similarities supporting specificity of the phenotype, but also some quantitative and qualitative differences: (i) with respect to WAT mass: in Adamts5 -/- -J mice lower sWAT and GON fat mass was found, which was significant after 2 weeks of cold exposure, but only showed a trend in the DIO model; (ii) with respect to BAT mass: in Adamts5 -/- -J mice no significant difference with WT mice was observed after 2 weeks of cold exposure nor in the DIO model; (iii) with respect to browning of WAT: in Adamts5 -/- -J mice higher expression of UCP-1 mRNA and protein was found after 2 weeks of cold exposure (mRNA: 1.5- to 2-fold as compared to 5-fold for Adamts5 -/- -P mice).
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