A xenograft transplantation in immune-deficient mice was performed, and the estimated stem cell ratio in sphere-cultured cells was 1 in 185 cells, whereas the estimated stem cell ratio in adherent-culture cells was 1 in 770; however, the difference in stem cell ratios did not reach statistical significance (Figure 1B , P = 0.07).
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ST6GALNAC1 plays important roles in enhancing cancer stem phenotypes of colorectal cancer via the Akt pathway.
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