TCF21 hypermethylation regulates renal tumor cell clonogenic proliferation and migration.

Although the difference in proliferation was not significant at any of the chosen time points, we observed a mild trend toward decreased proliferation in the pBABE‐TCF21 clones (Fig. 2 A).

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Initial analysis of migration using the chemotaxis‐based Boyden chamber assay on two pBABE‐mock clones (N1G4 and B6D10) and two pBABE‐TCF21 clones (5D2 and 9D12) showed a trend toward inhibition of migration in TCF21 ‐expressing cells (analysis repeated for N1G4 and 5D2 clones).

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